Effects of substrate concentration and temperature on rate of enzyme activity

The rate of a reaction is the amount of change (e.g. substrate broken down or product made) per unit of time. Enzyme reaction rate increases as substrate concentration increases, until an optimum when all active sites are full. Enzyme reaction rate increases with temperature due to more collisions, until an optimum when all active sites are full. Enzyme reaction rate decreases as temperature increases above the optimum, as the active site becomes denatured. Interpretation of graphs showing effects of substrate concentration and temperature on enzyme reaction rate.