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Comparing types of chromatography: including gas chromatography

Lesson 12 / 18

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I can identify features of different types of chromatography and describe how the techniques are used to separate and identify components of a mixture.

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Paper, thin layer (TLC), and gas chromatography (GC) can all distinguish between pure and impure substances. Chromatographic processes use a mobile and stationary phase. TLC and GC both use silica/alumina as a stationary phase. Thin layer chromatography (TLC) uses locating agents to develop chromatograms and aid analysis of them. Chromatograms from gas chromatography (GC) also provide details about the amount of each component in a sample. Retention factors (Rf) and retention times both provide quantitative information about a sample's components.

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